Bacterial community structure of sediments of the bizerte lagoon (Tunisia), a southern Mediterranean coastal anthropized lagoon.
Identifieur interne : 000092 ( Main/Exploration ); précédent : 000091; suivant : 000093Bacterial community structure of sediments of the bizerte lagoon (Tunisia), a southern Mediterranean coastal anthropized lagoon.
Auteurs : Olfa Ben Said [France] ; Marisol Go I-Urriza ; Monia El Bour ; Patricia Aissa ; Robert DuranSource :
- Microbial ecology [ 1432-184X ] ; 2010.
Descripteurs français
- KwdFr :
- ARN bactérien (génétique), ARN ribosomique 16S (génétique), Analyse de séquence d'ADN (MeSH), Bactéries (classification), Bactéries (génétique), Bactéries (isolement et purification), Banque de gènes (MeSH), Eau de mer (microbiologie), Hydrocarbures (MeSH), Hydrocarbures aromatiques polycycliques (analyse), Métaux lourds (analyse), Phylogenèse (MeSH), Polluants chimiques de l'eau (analyse), Polymorphisme de restriction (MeSH), Profilage d'ADN (MeSH), Région méditerranéenne (MeSH), Sédiments géologiques (microbiologie), Tunisie (MeSH).
- MESH :
- analyse : Hydrocarbures aromatiques polycycliques, Métaux lourds, Polluants chimiques de l'eau.
- génétique : ARN bactérien, ARN ribosomique 16S, Bactéries.
- isolement et purification : Bactéries.
- microbiologie : Eau de mer, Sédiments géologiques.
- Analyse de séquence d'ADN, Banque de gènes, Hydrocarbures, Phylogenèse, Polymorphisme de restriction, Profilage d'ADN, Région méditerranéenne, Tunisie.
English descriptors
- KwdEn :
- Bacteria (classification), Bacteria (genetics), Bacteria (isolation & purification), DNA Fingerprinting (MeSH), Gene Library (MeSH), Geologic Sediments (microbiology), Hydrocarbons (MeSH), Mediterranean Region (MeSH), Metals, Heavy (analysis), Phylogeny (MeSH), Polycyclic Aromatic Hydrocarbons (analysis), Polymorphism, Restriction Fragment Length (MeSH), RNA, Bacterial (genetics), RNA, Ribosomal, 16S (genetics), Seawater (microbiology), Sequence Analysis, DNA (MeSH), Tunisia (MeSH), Water Pollutants, Chemical (analysis).
- MESH :
- chemical , analysis : Metals, Heavy, Polycyclic Aromatic Hydrocarbons, Water Pollutants, Chemical.
- chemical , genetics : RNA, Bacterial, RNA, Ribosomal, 16S.
- chemical : Hydrocarbons.
- classification : Bacteria.
- genetics : Bacteria.
- isolation & purification : Bacteria.
- microbiology : Geologic Sediments, Seawater.
- DNA Fingerprinting, Gene Library, Mediterranean Region, Phylogeny, Polymorphism, Restriction Fragment Length, Sequence Analysis, DNA, Tunisia.
Abstract
In order to estimate how pollution affects the bacterial community structure and composition of sediments, chemical and molecular approaches were combined to investigate eight stations around the Bizerte lagoon. Terminal restriction fragment length polymorphism (T-RFLP) analysis of PCR-amplified 16S rRNA genes revealed that each station was characterized by a specific bacterial community structure. The combination of this data with those of chemical analysis showed a correlation between the bacterial fingerprint and the pollutant content, principally with hydrocarbon pollution. The composition of the bacterial community of two contrasted stations related to the pollution revealed sequences affiliated to alpha, beta, gamma, delta, epsilon subclass of the Proteobacteria, Actinobacteria, and Acidobacteria in both stations although in different extent. Gamma and delta subclass of the Proteobacteria were dominant and represent 70% of clones in the heavy-metal-contaminated station and 47% in the polyaromatic hydrocarbon (PAH)-contaminated. Nevertheless, most of the sequences found were unaffiliated to cultured bacteria. The adaptation of the bacterial community mainly to PAH compounds demonstrated here and the fact that these bacterial communities are mainly unknown suggest that the Bizerte lagoon is an interesting environment to understand the capacity of bacteria to cope with some pollutants.
DOI: 10.1007/s00248-009-9585-x
PubMed: 19789910
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000727
- to stream PubMed, to step Curation: 000723
- to stream PubMed, to step Checkpoint: 000718
- to stream Main, to step Merge: 000092
- to stream Main, to step Curation: 000092
Le document en format XML
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sortKey="Go I Urriza, Marisol" sort="Go I Urriza, Marisol" uniqKey="Go I Urriza M" first="Marisol" last="Go I-Urriza">Marisol Go I-Urriza</name></author><author><name sortKey="El Bour, Monia" sort="El Bour, Monia" uniqKey="El Bour M" first="Monia" last="El Bour">Monia El Bour</name></author><author><name sortKey="Aissa, Patricia" sort="Aissa, Patricia" uniqKey="Aissa P" first="Patricia" last="Aissa">Patricia Aissa</name></author><author><name sortKey="Duran, Robert" sort="Duran, Robert" uniqKey="Duran R" first="Robert" last="Duran">Robert Duran</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:19789910</idno><idno type="pmid">19789910</idno><idno type="doi">10.1007/s00248-009-9585-x</idno><idno type="wicri:Area/PubMed/Corpus">000727</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000727</idno><idno type="wicri:Area/PubMed/Curation">000723</idno><idno 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xml:lang="fr">France</country><wicri:regionArea>Equipe Environnement et Microbiologie-IPREM UMR 5254-IBEAS, Université de Pau et des Pays de l'Adour, Avenue de l'Université, BP 1155, 64013 Pau Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Pau</settlement></placeName></affiliation></author><author><name sortKey="Go I Urriza, Marisol" sort="Go I Urriza, Marisol" uniqKey="Go I Urriza M" first="Marisol" last="Go I-Urriza">Marisol Go I-Urriza</name></author><author><name sortKey="El Bour, Monia" sort="El Bour, Monia" uniqKey="El Bour M" first="Monia" last="El Bour">Monia El Bour</name></author><author><name sortKey="Aissa, Patricia" sort="Aissa, Patricia" uniqKey="Aissa P" first="Patricia" last="Aissa">Patricia Aissa</name></author><author><name sortKey="Duran, Robert" sort="Duran, Robert" uniqKey="Duran R" first="Robert" last="Duran">Robert Duran</name></author></analytic><series><title level="j">Microbial ecology</title><idno type="eISSN">1432-184X</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteria (classification)</term><term>Bacteria (genetics)</term><term>Bacteria (isolation & purification)</term><term>DNA Fingerprinting (MeSH)</term><term>Gene Library (MeSH)</term><term>Geologic Sediments (microbiology)</term><term>Hydrocarbons (MeSH)</term><term>Mediterranean Region (MeSH)</term><term>Metals, Heavy (analysis)</term><term>Phylogeny (MeSH)</term><term>Polycyclic Aromatic Hydrocarbons (analysis)</term><term>Polymorphism, Restriction Fragment Length (MeSH)</term><term>RNA, Bacterial (genetics)</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Seawater (microbiology)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Tunisia (MeSH)</term><term>Water Pollutants, Chemical 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community structure and composition of sediments, chemical and molecular approaches were combined to investigate eight stations around the Bizerte lagoon. Terminal restriction fragment length polymorphism (T-RFLP) analysis of PCR-amplified 16S rRNA genes revealed that each station was characterized by a specific bacterial community structure. The combination of this data with those of chemical analysis showed a correlation between the bacterial fingerprint and the pollutant content, principally with hydrocarbon pollution. The composition of the bacterial community of two contrasted stations related to the pollution revealed sequences affiliated to alpha, beta, gamma, delta, epsilon subclass of the Proteobacteria, Actinobacteria, and Acidobacteria in both stations although in different extent. Gamma and delta subclass of the Proteobacteria were dominant and represent 70% of clones in the heavy-metal-contaminated station and 47% in the polyaromatic hydrocarbon (PAH)-contaminated. Nevertheless, most of the sequences found were unaffiliated to cultured bacteria. The adaptation of the bacterial community mainly to PAH compounds demonstrated here and the fact that these bacterial communities are mainly unknown suggest that the Bizerte lagoon is an interesting environment to understand the capacity of bacteria to cope with some pollutants.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Aquitaine</li><li>Nouvelle-Aquitaine</li></region><settlement><li>Pau</li></settlement></list><tree><noCountry><name sortKey="Aissa, Patricia" sort="Aissa, Patricia" uniqKey="Aissa P" first="Patricia" last="Aissa">Patricia Aissa</name><name sortKey="Duran, Robert" sort="Duran, Robert" uniqKey="Duran R" first="Robert" last="Duran">Robert Duran</name><name sortKey="El Bour, Monia" sort="El Bour, Monia" uniqKey="El Bour M" first="Monia" last="El Bour">Monia El Bour</name><name sortKey="Go I Urriza, Marisol" sort="Go I Urriza, Marisol" uniqKey="Go I Urriza M" first="Marisol" last="Go I-Urriza">Marisol Go I-Urriza</name></noCountry><country name="France"><region name="Nouvelle-Aquitaine"><name sortKey="Ben Said, Olfa" sort="Ben Said, Olfa" uniqKey="Ben Said O" first="Olfa" last="Ben Said">Olfa Ben Said</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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